George M. Malacinski - Essentials of Molecular Biology
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Biological Sciences
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George M. Malacinski Indiana University David Freifelder Late of the University of California, San Diego
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Copyright 1998 by Jones and Bartlett Publishers, Inc.
All rights reserved. No part of the material protected by this copyright notice may be reproduced or utilized in any form, electronic or mechanical, including photocopying, recording, or by any information storage and retrieval system, without written permission from the copyright owner.
Library of Congress Cataloging-in-Publication Data Malacinski, George M. Essentials of molecular biology / George M. Malacinski, David Freifelder.3rd ed. p. cm. Freifelder's name appears first on the earlier edition. Includes bibliographical references (p. ) and index. ISBN 0-86720-860-0 (hardcover) 1. Molecular biology. I. Freifelder, David, 1935 II. Title. QH506.M368 1998 572.8dc21 97-44023 CIP
PRINTED IN THE UNITED STATES 02 01 00 99 9 8 7 6 5 4 3 2
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This edition of Essentials of Molecular Biology is dedicated to the memory of David Freifelder, who in his short lifetime, con veyed his love and appreciation of science to a wide range of students.
It is better to understand a little than to misunderstand a lot. Anatole France
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BRIEF CONTENTS
Prologue
xv
Preface
xvii
Introduction
1
Chapter 1 Welcome to Molecular Biology!
3
Part I The Structure of Proteins, Nucleic Acids, and Macromolecular Complexes
21
Chapter 2 Macromolecules
23
Chapter 3 Nucleic Acids
39
Chapter 4 The Physical Structure of Protein Molecules
67
Chapter 5 Macromolecular Interactions and the Structure of Complex Aggregates
87
Part II Function of Macromolecules
107
Chapter 6 The Genetic Material
109
Chapter 7 DNA Replication
131
Chapter 8 Transcription
163
Chapter 9 Translation
187
Chapter 10 Mutations, Mutagenesis, and DNA Repair
213
Part III Coordination of Macromolecular Function in Cells
249
Chapter 11 Regulation of Gene Activity in Prokaryotes
251
Chapter 12 Bacteriophage
281
Chapter 13 Regulation of Gene Activity in Eukaryotes
311
Part IV Experimental Manipulation of Macromolecules
349
Chapter 14 Plasmids and Transposons
351
Chapter 15 Recombinant DNA and Genetic Engineering: Molecular Tailoring of Genes
381
Chapter 16 Molecular Biology is Expanding its Reach
409
Appendix Chemical Principles Important for Understanding Molecular Biology
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