Lydiane Kyte - Plants from test tubes: an introduction to micropropagation
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Plants from test tubes: an introduction to micropropagation
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Acclaimed since its first appearance as the most practical guide to plant tissue culture and widely adopted as a textbook, this standard work is now even better. This expanded edition introduces new developments in biotechnology, such as genetic engineering and cell culture. It provides detailed recipes for propagating plants from more than 30 families. It explains clearly how to set up a propagating laboratory, from a hobbyists kitchen to an elaborate commercial enterprise.
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This edition is dedicated to the memory of Dr. Hudson Hartmann (19151994). His untiring efforts to promote good horticulture through his labors as professor and author and as mentor for the International Plant Propagators' Society have made a lasting impression on the state of the art.
Copyright 1996 by Timber Press, Inc. All rights reserved.
ISBN 0-88192-361-3
Printed in Hong Kong
Timber Press, Inc. The Haseltine Building 133 S.W. Second Avenue, Suite 450 Portland, Oregon 97204, U.S.A.
Library of Congress Cataloging-in-Publication Data
Kyte, Lydiane. Plants from test tubes: an introduction to micropropagation / Lydiane Kyte and John Kleyn.3rd ed. p. cm. Includes bibliographical references (p. ) and index. ISBN 0-88192-361-3 1. Plant micropropagationLaboratory manuals. 2. Plant tissue cultureLaboratory manuals. I. Kleyn, John G. II. Title SB123.6.K98 1996 631.5'3dc20 96-33972 CIP
Page 5
CONTENTS
Foreword
by Michael E. Kane
7
Preface
9
Section I The Basics of Tissue Culture
Chapter 1 Overview
13
Chapter 2 The Botanical Basis for Tissue Culture
21
Chapter 3 History
27
Chapter 4 The LaboratoryFacilities and Supplies
35
Chapter 5 Media Ingredients
60
Chapter 6 Media Preparation
76
Chapter 7 Explants and Their Preparation
84
Chapter 8 The Transfer Room
90
Chapter 9 The Growing Room
101
Chapter 10 Hardening Off
108
Chapter 11 Dealing with Biological Contaminants
113
Chapter 12 Business
137
Chapter 13 Biotechnology
146
Section II Culture Guide to Selected Plants
Ferns
169
Conifers
170
Monocotyledons
171
Dicotyledons
183
Formula Comparison Chart
206
Appendix A Metric Conversions
208
Appendix B The MicroscopeIntroduction, Use, and Care
210
Appendix C Professional Organizations
214
Appendix D Suppliers
215
Glossary
219
Bibliography
227
Index
239
Page 7
FOREWORD
More than 30 years have elapsed since George Morel first proposed the application of plant tissue culture for the commercial clonal propagation of virus-free orchids. Since that time, in vitro propagation, or micropropagation as it is commonly known, has evolved into a competitive, worldwide industry that produces 250 million plants annually. In the United States alone there are approximately 75 large commercial micropropagation laboratories. Production has long centered on high-value ornamental crops, such as tropical foliage plants and orchids. More recently, commercial micropropagation of woody landscape plants, fruits, vegetables, and specialty crops has become increasingly common as laboratories strive to fill new markets. Undoubtedly, micropropagation has proven to be a commercially viable method for plant production and an important tool in the application of plant biotechnology in agriculture. Scientific advancements are continually being made in areas such as synthetic seed technology and automated micropropagation systems development. However, the information being generated is often available only in very expensive and specialized journals, symposium proceedings, and advanced text books. Unfortunately, the number of affordable texts that provide a practical but thorough overview of the micropropagation process have been few. For those who ask, What is micropropagation and how can I do it?, the choices have been limited.
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