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S. Suzanne Nielsen - Food Analysis Laboratory Manual

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S. Suzanne Nielsen Food Analysis Laboratory Manual
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Food Science Text Series Series Editor Dennis R Heldman Heldman Associates - photo 1
Food Science Text Series
Series Editor
Dennis R. Heldman
Heldman Associates, Mason, Ohio, USA

For other titles published in this series, go to www.springer.com/series/5999

The Food Science Text Series provides faculty with the leading teaching tools. The Editorial Board has outlined the most appropriate and complete content for each food science course in a typical food science program and has identified textbooks of the highest quality, written by the leading food science educators. Series Editor Dennis R. Heldman, Professor, Department of Food, Agricultural, and Biological Engineering, The Ohio State University. Editorial Board; John Coupland, Professor of Food Science, Department of Food Science, Penn State University, David A. Golden, Ph.D., Professor of Food Microbiology, Department of Food Science and Technology, University of Tennessee, Mario Ferruzzi, Professor, Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Richard W. Hartel, Professor of Food Engineering, Department of Food Science, University of Wisconsin, Joseph H. Hotchkiss, Professor and Director of the School of Packaging and Center for Packaging Innovation and Sustainability, Michigan State University, S. Suzanne Nielsen, Professor, Department of Food Science, Purdue University, Juan L. Silva, Professor, Department of Food Science, Nutrition and Health Promotion, Mississippi State University, Martin Wiedmann, Professor, Department of Food Science, Cornell University, Kit Keith L. Yam, Professor of Food Science, Department of Food Science, Rutgers University

S. Suzanne Nielsen
Food Analysis Laboratory Manual 3rd ed. 2017
S Suzanne Nielsen Department of Food Science Purdue University West - photo 2
S. Suzanne Nielsen
Department of Food Science, Purdue University, West Lafayette, Indiana, USA
ISSN 1572-0330 e-ISSN 2214-7799
Food Science Text Series
ISBN 978-3-319-44125-2 e-ISBN 978-3-319-44127-6
https://doi.org/10.1007/978-3-319-44127-6
Library of Congress Control Number: 2017942968
corrected publication 2019
Springer International Publishing 2017
This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed.
The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use.
The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

This Springer imprint is published by Springer Nature

The registered company is Springer International Publishing AG

The registered company address is: Gewerbestrasse 11, 6330 Cham, Switzerland

Preface and Acknowledgments

This laboratory manual was written to accompany the textbook, Food Analysis , fifth edition. The laboratory exercises are tied closely to the text and cover 21 of the 35 chapters in the textbook. Compared to the second edition of this laboratory manual, this third edition contains four introductory chapters with basic information that compliments both the textbook chapters and the laboratory exercises (as described below). Three of the introductory chapters include example problems and their solutions, plus additional practice problems at the end of the chapter (with answers at the end of the laboratory manual). This third edition also contains three new laboratory exercises, and previous experiments have been updated and corrected as appropriate. Most of the laboratory exercises include the following: background, reading assignment, objective, principle of method, chemicals (with CAS number and hazards), reagents, precautions and waste disposal, supplies, equipment, procedure, data and calculations, questions, and resource materials.

Instructors using these laboratory exercises should note the following:
  1. Use of Introductory Chapters:
    • Chap. 1, Laboratory Standard Operating Procedures recommended for students prior to starting any food analysis laboratory exercises

    • Chap. 2, Preparation of Reagents and Buffers includes definition of units of concentrations, to assist in making chemical solutions

    • Chap. 3, Dilution and Concentration Calculations relevant for calculations in many laboratory exercises

    • Chap. 4, Use of Statistics in Food Analysis relevant to data analysis

  2. Order of Laboratory Exercises: The order of laboratory exercises has been changed to be fairly consistent with the reordering of chapters in the textbook, Food Analysis , fifth edition (i.e., chromatography and spectroscopy near the front of the book). However, each laboratory exercise stands alone, so they can be covered in any order.

  3. Customizing Laboratory Procedures: It is recognized that the time and equipment available for teaching food analysis laboratory sessions vary considerably between schools, as do student numbers and their level in school. Therefore, instructors may need to modify the laboratory procedures (e.g., number of samples analyzed, replicates) to fit their needs and situation. Some experiments include numerous parts/methods, and it is not assumed that an instructor uses all parts of the experiment as written. It may be logical to have students work in pairs to make things go faster. Also, it may be logical to have some students do one part of the experiment/one type of sample and other students to another part of the experiment/type of sample.

  4. Use of Chemicals: The information on hazards and precautions in the use of the chemicals for each experiment is not comprehensive but should make students and a laboratory assistant aware of major concerns in handling and disposing of the chemicals.

  5. Reagent Preparation: It is recommended in the text of the experiments that a laboratory assistant prepare many of the reagents, because of the time limitations for students in a laboratory session. The lists of supplies and equipment for experiments do not necessarily include those needed by the laboratory assistant in preparing reagents for the laboratory session.

  6. Data and Calculations: The laboratory exercises provide details on recording data and doing calculations. In requesting laboratory reports from students, instructors will need to specify if they require just sample calculations or all calculations.

Even though this is the third edition of this laboratory manual, there are sure to be inadvertent omissions and mistakes. I will very much appreciate receiving suggestions for revisions from instructors, including input from lab assistants and students.

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