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Carol Ann Kearns - Techniques for Pollination Biologists

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Techniques for Pollination Biologists is the first book to incorporate all techniques published in the pollination literature as well as unpublished methods compiled from practicing pollination biologists. The bibliography includes 1,200 references from more than 200 journals, plus books and previously unpublished materials. Appendices list sources for all the equipment and chemicals needed. This book presents the newest techniques such as fluorescence microscopy to examine pollen tubes, high-pressure liquid chromatography for nectar analysis, and using particle counters to count pollen grains and nuclear magnetic resonance for floral odor analysis. In addition to these sophisticated methods, basic techniques are described for labeling plants, manipulating flowers, marking or excluding, and designing simple but elegant experiments with small budgets. The book also examines potential pitfalls for pollination studies and offers cautionary advice about designing and implementing different types of pollination experiments.

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Carol Ann Kearns
Techniques for Pollination Biologists

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title Techniques for Pollination Biologists author Kearns Carol - photo 1
title:Techniques for Pollination Biologists
author:Kearns, Carol Ann.; Inouye, David William
publisher:University Press of Colorado
isbn10 | asin:0870812815
print isbn13:9780870812811
ebook isbn13:9780585004495
language:English
subjectPollination--Research--Technique.
publication date:1993
lcc:QK926.K425 1993eb
ddc:582/.01662
subject:Pollination--Research--Technique.
Page iii

Techniques
for Pollination Biologists
Carol Ann Kearns
Environmental, Population, and Organismic Biology
University of Colorado and Mountain Research Station, University of Colorado
David William Inouye
Departments of Zoology and of Botany
University of Maryland and Rocky Mountain Biological Laboratory
UNIVERSITY PRESS OF COLORADO
Page iv

Copyright 1993 by the University Press of Colorado

P.O. Box 849

Niwot, Colorado 80544

All rights reserved.

The University Press of Colorado is a cooperative publishing enterprise supported, in part, by Adams State College, Colorado State University, Fort Lewis College, Mesa State College, Metropolitan State College of Denver, University of Colorado, University of Northern Colorado, University of Southern Colorado, and Western State College.

Library of Congress Cataloging-in-Publication Data

Kearns, Carol Ann, 1955

Techniques for pollination biologists / Carol Ann Kearns, David William Inouye.

p. cm.

Includes bibliographical references and index.

ISBN 0-87081-279-3 (alk. paper). ISBN 0-87081-281-5 (pbk., alk. paper)

1. Pollination Research Technique. I. Inouye, David William, 1950 . II. Title.

QK926.K425 1993

582'.01662 dc20

CIP

93-14994

The paper used in this publication meets the minimum requirements of the American National Standard for Information SciencesPermanence of Paper for Printed Library Materials. ANSI Z39.481984

10 9 8 7 6 5 4 3 2

Page v

Contents
Preface
xv
Acknowledgments
xvii
1. Introduction
1
2. Plants
11
A. Collecting and Preserving Plants
11
B. Marking or Tagging Plants
12
C. Preventing Visitation
13
D. Abiotic Pollination
19
1. Hydrophily
19
2. Anemophily
20
E. Flowering Phenology
22
F. Seeds
29
1. Seed Predation
29
2. Seed Viability
30
G. Suggestions for Planning Studies
33
3. Flowers
35
A. Marking or Tagging Flowers
35
B. Preventing Visitation
37
C. Morphological Measurements
39
1. Floral Measurements And Intercorrelations
39
a. Corolla tubes
41
b. Other floral measurements
42
c. Flower orientation
44
2. Inflorescence Structure
45

Page vi

3. Energy Content
46
D. Floral Fragrance
47
E. Flower Color
57
1. Visual Appearance to Humans
58
2. Measuring Visible Light Reflectance Levels
58
3. Measuring Spectral Reflectance
59
4. Ultraviolet Reflectance Patterns
63
F. Gynoecium
65
1. Stigma Secretions
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