Meyer - Pitfalls and Errors of HPLC in Pictures
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![Further Titles for Chromatographers S Kromidas ed HPLC Made to Measure - photo 1](/uploads/posts/book/220246/images/cover_fmt.jpg)
Further Titles for Chromatographers
S. Kromidas (ed.)
HPLC Made to Measure
A Practical Handbook for Optimization
2006
ISBN 3-527-31377-X
S. Kromidas
More Practical Problem Solving in HPLC
2005
ISBN 3-527-31113-0
V. R. Meyer
Practical High-Performance Liquid Chromatography
2010
ISBN 978-0-470-68218-0 or 978-0-470-68217-3
S. Kromidas
Practical Problem Solving in HPLC
2000
ISBN 3-527-29842-8
P. C. Sadek
Troubleshooting HPLC Systems
A Bench Manual
2000
ISBN 0-471-17834-9
U. D. Neue
HPLC Columns
Theory, Technology, and Practice
1997
ISBN 0-471-19037-3
L. R. Snyder, J. J. Kirkland, J. L. Glajch
Practical HPLC Method Development
1997
ISBN 0-471-00703-X
The Authors
Dr. Veronika R. Meyer
EMPA St. Gallen
Lerchenfeldstrasse 5
9014 St. Gallen
Switzerland
e-mail: veronika.meyer@empa.ch
All books published by Wiley-VCH are carefully produced. Nevertheless, authors, editors, and publisher do not warrant the information contained in these books, including this book, to be free of errors. Readers are advised to keep in mind that statements, data, illustrations, procedural details or other items may inadvertently be inaccurate.
Library of Congress Card No.: applied for A catalogue record for this book is available from the British Library.
Bibliographic information published by the Deutsche Nationalbibliothek
The Deutsche Nationalbibliothek lists this publication in the Deutsche Nationalbibliografie; detailed bibliographic data are available on the Internet at .
2013 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
All rights reserved (including those of translation into other languages). No part of this book may be reproduced in any form by photoprinting, microfilm, or any other means nor transmitted or translated into a machine language without written permission from the publishers. Registered names, trademarks, etc. used in this book, even when not specifically marked as such, are not to be considered unprotected by law.
Print ISBN: 978-3-527-33293-9
ePDF ISBN: 978-3-527-65913-5
ePub ISBN: 978-3-527-65912-8
mobi ISBN: 978-3-527-65911-1
oBook ISBN: 978-3-527-65910-4
1 wort = 1 millibild
(A picture says more than a thousand words)
Graffiti on the Baltzerstrasse in Bern
Preface
Errors are a common companion of all human activity, including work in the laboratory. Yet it is a great pity if erroneous results are produced with great effort and by using expensive instruments and demanding procedures. Therefore a book about sources of errors in high performance liquid chromatography, one of today's most widely used analytical methods, is not superfluous. Maybe the topic is not welcomed enthusiastically but I hope I have found a design which encourages reading and thinking.
In conception, at least, possible problems can be divided into two categories. Errors are troublesome opponents of accurate and precise analytical results which, however, can be understood; we need to remember and to anticipate them. In contrast, pitfalls are totally unexpected intruders and the secret behind them is difficult to discover. The worst are those which are not detected but which affect the result anyway. Nevertheless, the book does not distinguish between the two types. The readers decide how they classify them. With increasing experience in HPLC it should become easier to avoid the pitfalls.
The third edition could be expanded with new examples and proposals. Many people helped me with examples, hints or ideas on how to improve the text and figures. I want to thank all of them. Special thanks to the publisher who supports the idea of a picture book, not for children but for novices and experts in the analytical laboratory. I hope that the book will be a useful aid in daily laboratory work thanks to intelligible explanations and lucid illustrations.
Veronika R. Meyer
St. Gallen, August 2012
Introduction
This book is not an introductory text to HPLC and also not a troubleshooting guide of the kind what shall I do if my instrument does not work?. It does not replace such books but is intended to complement them. Some texts which, according to my personal opinion, are very useful and should therefore be present in the HPLC laboratory are listed on the next page.
Now this book on your desk is a picture book. The figures are at least as important as the texts; sometimes more information can be found in them than could be given in the short descriptions. It is possible, and in principle recommended, to study all the pages in sequence from beginning to end. This method guarantees that one learns about errors which are uncommon and unexpected. On the other hand each pair of pages is limited to one topic, linked to other pages by arrows only, and can therefore be studied in isolation. The index at the end of the book can help you find the right pages when a problem occurs, although it must be stated once again that quick troubleshooting advice is not usually provided.
The book is divided into three parts:
Part I briefly presents some basic facts about HPLC. Many topics may be absent because this is not a textbook, but the matter presented is of utmost relevance in HPLC. Thus the topics discussed should act as reminders and be used for revision. Whoever understands Part I knows a lot about HPLC more than it seems at first glance.
Part II lists the pitfalls and sources of error. They are in a logical sequence, as far as this is possible, following the flow path in an HPLC instrument, from the preparation of the mobile phase to data evaluation. The list is somewhat arbitrary, and not all errors are of equal importance with regard to their possible consequences. It would, however, be dangerous to distinguish between grave and harmless errors. A minute error can cause much damage under special circumstances.
Part III gives some hints on what can be done to avoid errors. Again this synopsis is very heterogeneous in character. This does not diminish its value, of course.
Incompleteness is an inevitable feature of this book. I am grateful for all hints on other pitfalls and sources of error or on how to avoid them.
Recommended Texts
Veronika R. Meyer
Practical High Performance Liquid Chromatography
Wiley, Chichester
5th edition 2010
John W. Dolan and Lloyd R. Snyder
Troubleshooting LC Systems
Humana Press, New Jersey
1989
Paul C. Sadek
Troubleshooting HPLC Systems: A Bench Manual
Wiley-Interscience, New York
2000
Stavros Kromidas
Practical Problem Solving in HPLC
Wiley-VCH, Weinheim
2000
Stavros Kromidas
More Practical Problem Solving in HPLC
Wiley-VCH, Weinheim
2004
Lloyd R. Snyder, Joseph J. Kirkland and Joseph L. Glajch
Practical HPLC Method Development
Wiley-Interscience, New York
2nd edition 1997
Norman Dyson
Chromatographic Integration Methods
Royal Society of Chemistry, London
2nd edition 1998
Part I
Fundamentals
In chromatography, a physical separation method, the components of a mixture are partitioned between two phases. One of the phases stays in its place and is called the stationary phase, whereas the other moves in a definite direction and is called the mobile phase.
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