Carmen V. Sciortino - Atlas of Clinically Important Fungi

1. Chapter 04
2. Chapter 05
3. Chapter 06
4. Chapter 07
5. Chapter 08
6. Chapter 09

Carmen V. Sciortino, Jr.

Division of Infectious Diseases
University of Louisville School of Medicine, and
Robley Rex Veterans Healthcare Medical Center
Louisville, kentucky, USA

Copyright 2017 by John Wiley & Sons, Inc. All rights reserved

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Library of Congress CataloginginPublication Data

Names: Sciortino, Carmen V., Jr., 1953
Title: Atlas of clinically important fungi / Carmen V. Sciortino, Jr.
Description: Hoboken, New Jersey : John Wiley & Sons, Inc., [2017] | Includes bibliographical references and index.
Identifiers: LCCN 2016036788 (print) | LCCN 2016046904 (ebook) | ISBN 9781119069669 (cloth) | ISBN 9781119069676 (pdf) | ISBN 9781119069683 (epub)
Subjects: LCSH: FungiIdentification. | FungiAtlases. | Materia medica, VegetableAtlases.
Classification: LCC QK603 .S344 2017 (print) | LCC QK603 (ebook) | DDC 579.5dc23
LC record available at https://lccn.loc.gov/2016036788

This book is dedicated in loving memory of my Father and to the many other War Veterans that served our country.

Although there are many texts that provide quality information for the identification of fungi, I have found that medical technologists rarely have time to read the text. Hence, most are rushed and seek morphological information that helps guide them to the identification of fungi. In other words, one picture is worth a thousand words. This atlas is a reference for those who have a basic understanding of mycology and wish to identify an organism with just culture morphology and a lactophenol cotton blue (LPCB) stain. It is written in simplified, common terms rather than in strict mycological terms to avoid the need to consult a glossary.

I have attempted to show many photographs without extensive morphological descriptions. Photographs show most structures that the technologist can correlate with their own preparations. For this reason, numerous color photographs are included so that technologists will have sufficient reference photos for identifying the various morphologies of a single organism. Organism photographs begin with the macroscopic colony views followed by the microscopic views. Also included for some microorganisms are clinical pathology photographs demonstrating how the organism appears in human tissues.

Special thanks go to James Jim Lay, Mary Kemper, Karen Petach, Gerald Doug Thompson, Derli Yung, Mark Constant, Kimberly Pittman, Mark Heckman, and Charles Chuck Cornett, a great team to work with over the years. James Jim Snyder is much appreciated for providing several of the fungal isolates and his valuable consultations. A special thanks to the pathologists, Larry Rubel and Saad Shaheen, for their consultations. The University of Texas Health Science Center Fungus Testing Laboratory, San Antonio, TX, kindly provided the Conidiobolus isolate from Dr M. Kimuras laboratory.

BABlood agarBHIBrain heart infusion agarCMACorn meal agarGMSGrocotts methenamine silver stainH&EHematoxylin and eosinLPCBLactophenol cotton bluePASPeriodic acidSchiff stainPDAPotato dextrose agarSABDXSabouraud dextrose agar Emmons modification

Growth: slow, beginning glabrous, developing into fuzzy appearance in later culture. May also appear folded and convoluted, giving a molar toothlike colony.

Nocardia (white, pink to orange) (see figures in )

Rhodococcus equi (coral) (see figures in )

Streptomyces (white, cream, brown, black) (see figures in )

Rhodococcus equi (see figures in )

Nocardia (see figures in )

Candida species (see figures in )

Cryptococcus (see figures in ) (for further information see reference 64 in Additional Reading)

Pichia (Hansenula) anomala (see figures in )

Malassezia (see figures in ) (for further information see reference 65 in Additional Reading)

Trichosporon (see figures in ) (for further information see reference 63 in Additional Reading)

Prototheca Algae (see figures in )

Cryptococcus (yellow pigment on Sabouraud dextrose) (see figures in ) (for further information see reference 64 in Additional Reading)

Rhodotorula (pink or coral pigment) (see figures in )

Coccidioides immitis (see figures in ) (for further information see references 10 and 11 in Additional Reading)

Blastoschizomyces (see figures in ) (for further information see reference 61 in Additional Reading)

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