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P. E. Nielsen - Peptide Nucleic Acids: Protocols and Applications (Horizon Bioscience)

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Peptide Nucleic Acids, Second Edition has been extensively revised, updated, and enlarged to contain many new chapters covering the most recent topics and applications in this fast-moving field. The book contains state-of-the-art protocols and applications on all aspects of peptide nucleic acids. Concepts are clearly explained with each chapter containing concise background information. Written by leading experts in the field, the book is an invaluable and complete reference work on this novel and exciting area.

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title Peptide Nucleic Acids Protocols and Applications 2Nd Ed - photo 1
title:Peptide Nucleic Acids : Protocols and Applications 2Nd Ed. /
author:Nielsen, Peter E.
publisher:Taylor & Francis Routledge
isbn10 | asin:0954523245
print isbn13:9780954523244
ebook isbn13:9780203508558
language:English
subjectNucleic acids, Peptide Nucleic Acids--Laboratory Manuals.
publication date:2004
lcc:QD431.P47 2004eb
ddc:572.8
subject:Nucleic acids, Peptide Nucleic Acids--Laboratory Manuals.

Page a-2

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Page i

PEPTIDE NUCLEIC ACIDS

Protocols and Applications

Second Edition

Edited by

Peter E.Nielsen
The Panum Institute, Copenhagen

Picture 2

horizon bioscience

Page ii

This edition published in the Taylor & Francis e-Library, 2005.


To purchase your own copy of this or any of Taylor & Francis or Routledges collection of thousands of eBooks please go to www.eBookstore.tandf.co.uk.

Copyright 2004
Horizon Bioscience
32 Hewitts Lane
Wymondham
Norfolk NR 18 0JA
U.K.

www.horizonbioscience.com

British Library Cataloguing-in-Publication Data

A catalogue record for this book is available from the British Library

ISBN 0-203-50855-6 Master e-book ISBN

ISBN 0-203-61705-3 (OEB Format)

ISBN: 0-9545232-4-5 (Print Edition)

Description or mention of instrumentation, software, or other products in this book
does not imply endorsement by the author or publisher. The author and publisher do
not assume responsibility for the validity of any products or procedures mentioned
or described in this book or for the consequences of their use.

All rights reserved. No part of this publication may be reproduced, stored in a retrieval
system, or transmitted, in any form or by any means, electronic, mechanical,
photocopying, recording or otherwise, without the prior permission of the publisher.
No claim to original U.S. Government works.

Page iii

Contents

Contents

iii

Contributors

v

Preface

vii

Chapter 1

An Introduction to PNA
Peter E.NielsenandMichael Egholm

Chapter 2

PNA Synthesis by Boc Chemistry
Troels Koch

Chapter 3

Synthesis of PNA Oligomers by Fmoc Chemistry
Ralph Casale,Ivar S.JensenandMichael Egholm

Chapter 4

Thermodynamics of PNA Interactions
Tommi Ratilainen,Anders HolmnandBengt Nordn

Chapter 5

Peptide Nucleic Acid Targeting of Double-Stranded DNA
Thomas Bentin,H.Jakob LarsenandPeter E.Nielsen

Chapter 6

PNA Beacons and Fluoroprobes
Bhaskar Datta,Miaomiao WangandBruce A.Armitage

Chapter 7

Vector Tagging via PNA
Alex G.Rebuffat,Alessio G.BernasconiandBrigitte M.Frey

Chapter 8

PCR Clamping
Henrik rum

Chapter 9

PNA Directed Genome Rare Cutting: New Developments
Maxim D.Frank-KamenetskiiandVadim V.Demidov

Page iv

Chapter 10

PNA Openers for Duplex DNA: Basic Facts, Fine Tuning and Emerging Applications
Vadim V.Demidov

Chapter 11

Combined Use of PNA and DNA for Fluorescence In Situ Hybridization (FISH)
Kirsten Vang Nielsen,Sven Mller,Tim Svenstrup Poulsen,Susanne GabsandAndreas Schnau

Chapter 12

Production of PNA-Arrays for Nucleic Acid Detection
Anette Jacob,Ole Brandt,Simone Wrtz,Achim Stephan,Martina SchnlzerandJrg D.Hoheisel

Chapter 13

Antisense in Eukaryotic Systems
David R.Corey

Chapter 14

Antisense Effects in Escherichia coli
Liam Good,Rikard DryseliusandPeter E.Nielsen

Appendix

Index

Page v

Contributors

Bruce A.Armitage

Department of Chemistry

Carnegie Mellon University

4400 Fifth Avenue

Pittsburgh PA 152133890, USA

army@cyrus.andrew.cmu.edu

Thomas Bentin

Center for Biomolecular Recognition

Institute for Medical Biochemistry and Genetics, Dept. B

University of Copenhagen

The Panum Institute

Blegdamsvej 3c

DK-2200 N Copenhagen, Denmark

Alessio G.Bernasconi

Division of Nephrology and Hypertension

Departement of Clinical Research

University of Berne

Freiburgstrasse

CH-3010 Inselspital Berne, Switzerland

Ole Brandt

Deutsches Krebsforschungszentrum

Im Neuenheimer Feld 580

D-69120 Heidelberg, Germany

o.brandt@dkfz.de

Ralph Casale

Applied Biosystems

15 DeAngelo Drive

Bedford MA 01730, USA

CasaleRA@appliedbiosystems.com

David R.Corey

Departments of Pharmacology and Biochemistry

University of Texas Southwestern Medical

Center at Dallas

5323 Harry Hines Blvd.

Dallas TX 753909041, USA

david.corey@utsouthwestern.edu

Bhaskar Datta

Department of Chemistry

Carnegie Mellon University

4400 Fifth Avenue

Pittsburgh PA 152133890, USA

Vadim V.Demidov

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