Loic P. Deleyrolle - Neural Progenitor Cells: Methods and Protocols

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For over 35 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice. These hallmark features were introduced by series editor Dr. John Walker and constitute the key ingredient in each and every volume of the Methods in Molecular Biology series. Tested and trusted, comprehensive and reliable, all protocols from the series are indexed in PubMed.

Cover Illustration Caption: For more details, see Figure 2D in Chapter 11.

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The discovery in the early 1990s of stem and progenitor cells in the adult mammalian central nervous system challenged the long-standing no new neuron dogma and opened the door to the potential for cell replacement therapeutic modalities. The road from discovery to clinical applications is long and very complex, requiring rigorous steps involving standardized and precise procedures. This book is a collection of practical articles describing techniques used to study neural stem and progenitor cells as well as the brain microenvironment. This collection, which features a wide range of technological advances in the study of neural stem cells, highlights the promises of stem cell-based therapeutic applications for central nervous system ailments.

I would like to express my sincere appreciation to all the authors for their valuable contributions to this volume, sharing their expertise and knowledge as well as providing practical protocols that will assist the next generation of neuroscientists as they develop innovative experimental paradigms and progress toward therapeutic applications in the field of neurobiology.