LAST LOOK
Microbiology
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LAST LOOK
Microbiology
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Largest Medical Education Institute in the Country
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Published by Delhi Academy of Medical Sciences (P) Ltd.
HEAD OFFICE
Delhi Academy of Medical Sciences (P.) Ltd.
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Near Karol Bagh Metro Station,
New Delhi-110 005 Phone : 011-4009 4009
http://www.damsdelhi.com Email: info@damsdelhi.com
ISBN :
First Published 1999, Delhi Academy of Medical Sciences 2018 DAMS Publication
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The agents of human infectious diseases are bacteria, fungi (yeasts and molds), protozoa, helminths (worms), and viruses. Bacterial cells have a prokaryotic nucleus, whereas human, fungal, protozoan, and helminth cells have a eukaryotic nucleus. Viruses are not cells and do not have a nucleus.
All cells contain both DNA and RNA, whereas viruses contain either DNA or RNA, but not both.
Bacterial and fungal cells are surrounded by a rigid cell wall, whereas human, protozoan, and helminth cells have a flexible cell membrane.
The bacterial cell wall contains peptidoglycan, whereas the fungal cell wall contains chitin.
Comparison of Conjugation, Transduction, and Transformation
Transfer Procedure | Process | Type of Cells Involved | Nature of DNA Transferred |
Conjugation | DNA transferred from one bacterium to another | Prokaryotic | Chromosomal or plasmid |
Transduction | DNA transferred by a virus from one cell to another | Prokaryotic | Any gene in generalized transduction; only certain genes in specialized transduction |
Transformation | Purified DNA taken up by a cell | Prokaryotic or eukaryotic (e.g. human) | Any DNA |
Members of the Normal Flora | Anatomic Location |
Bacteroides species | Colon, throat, vagina |
Candida albicans | Mouth, colon, vagina |
Clostridium species | Colon |
Corynebacterium species (diphtheroids) | Nasopharynx, skin, vagina |
Enterococcus faecalis | Colon |
Escherichia coli and other coliforms | Colon, vagina, outer urethra |
Gardnerella vaginalis | Vagina |
Haemophilus species | Nasopharynx, conjunctiva |
Lactobacillus species | Mouth, colon, vagina |
Neisseria species | Mouth, nasopharynx |
Propionibacterium acnes | Skin |
Pseudomonas aeruginosa | Colon, skin |
Staphylococcus aureus | Nose, skin |
Staphylococcus epidermidis | Skin, nose, mouth, vagina, urethra |
Viridans streptococci | Mouth, nasopharynx |
Bacterial diseases transmitted by insects:
Bacterium | Insect | Reservoir | Disease |
Gram negative rods |
Yersinia pestis | Rat fleas | Rodents (e.g. rats and prairie dogs) | Plague |
Francisella tularensis | Ticks (Dermacentor) | Many animals (e.g. rabbits) | Tularemia |
Spirochaetes |
Borrelia burgdorferi | Ticks (brodes) | Mice | Lyme disease |
Borrelia recurrentis | Lice | Humans | Relapsing fever |
Rickettsiae |
Rickettsia rickettsii | Ticks (Demacentor) | Dogs, rodents, and ticks (Dermacentor) | Rocky Mountain spotted fever |
Rickettsia prowazekii | Lice | Humans | Epidemic typhus |
Ehrlichia chaffeensis | Ticks (Dermacentor, brodes) | Dogs | Ehrilichiosis |
Virulence Factors:
Organism | Virulence factors | Used in Vaccine | Comments |
Gram-positive cocci |
Streptococcus pneumoniae | Polysaccharide capsule | Yes | Determines serotype |
Streptococcus pyogenes | M protein | No | Determines serotype1 |
Streptococcus aureus | Protein A | No | Binds to Fc region of IgG, which prevents activation of complement |
Gram-negative cocci |
Neisseria meningitidis | Polysaccharide capsule | Yes | Determines serotype |
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