Jeffrey Coleman - Fusarium wilt: Methods and Protocols

For over 35 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice. These hallmark features were introduced by series editor Dr. John Walker and constitute the key ingredient in each and every volume of the Methods in Molecular Biology series. Tested and trusted, comprehensive and reliable, all protocols from the series are indexed in PubMed.

For further volumes: http://www.springer.com/series/7651

This Humana imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature.

The registered company address is: 1 New York Plaza, New York, NY 10004, U.S.A.

Fusarium wilt diseases are primarily caused by members of the Fusarium oxysporum species complex (FOSC) and are found worldwide. These filamentous phytopathogenic fungi are able to infect over 120 genera of plants, including tomato, banana, cucurbits, melon, cotton, and a wide range of fruits and vegetables. In addition to their broad host range, these fungi have many interesting biological aspects that have been the focus of investigation; in particular they have been well established to harbor additional chromosomes (i.e., the accessory genome) that confer many isolate-specific traits, including host specificity and niche adaptation. The unique characteristics of members of the FOSC have prompted researchers to develop and optimize several molecular procedures to study their biology. In this volume, a collection of molecular protocols detailing some of the most common and modern techniques for use with these fungi is presented. These methods range from the initial isolation and molecular-based identification to further specific protocols involving genome characterization, generation of mutants, and characterization of interactions with other organisms including host plants. This volume could serve as a valuable laboratory resource for mycologists, plant pathologists, microbiologists, geneticists, and others that have an interest in the FOSC or closely related fungi.