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John Whittall - Applied Biocatalysis: The Chemists Enzyme Toolbox

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Applied Biocatalysis: The Chemists Enzyme Toolbox: summary, description and annotation

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Provides clear and comprehensive coverage of recently developed applied biocatalysis for synthetic organic chemists with an emphasis to promote green chemistry in pharmaceutical and process chemistry

This book aims to make biocatalysis more accessible to both academic and industrial synthetic organic chemists. It focuses on current topics within the applied industrial biocatalysis field and includes short but detailed experimental methods on timely novel biocatalytic transformations using new enzymes or new methodologies using known enzymes. The book also features reactions that are expanding and making the enzyme toolbox available to chemistsproviding readers with comprehensive methodology and detailed key sourcing information of a wide range of enzymes.

Chapters in Applied Biocatalysis: The Chemists Enzyme Toolkit are organized by reaction type and feature a short introductory section describing the current state of the art for each example. Much of the book focuses on processes for which the enzymes are readily available so that organic chemists can synthesize appropriate quantities of chemicals with available materials in a standard chemical laboratory. Advanced methods are included to present examples of new enzymes that might encourage collaboration with suppliers or academic groups and that will educate chemists of rapidly expanding future possibilities.

  • Focuses on current topics within the applied industrial biocatalysis field
  • Offers experimental methods on novel biocatalytic transformations using new enzymes or new methodology using known enzymes
  • Covers the hot topics of enzyme and chemoenzymatic cascades and biocatalysis in flow
  • Edited by noted experts from both academia and industry with years of experience in the field of biocatalysisparticularly, the industrial applications of enzymes

Written for synthetic organic chemists working in all industries but especially the pharmaceutical industry and for those in academia with an eye for biocatalysis, Applied Biocatalysis: The Chemists Enzyme Toolkit will also benefit academic groups in chemistry and related sciences that are using enzymes for synthetic purposes, as well as those working in the area of enzymology and molecular biology.

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Table of Contents List of Tables Chapter 2 Chapter 3 Chapter 4 Chapter - photo 1
Table of Contents
List of Tables
  1. Chapter 2
  2. Chapter 3
  3. Chapter 4
  4. Chapter 5
  5. Chapter 6
  6. Chapter 8
  7. Chapter 9
  8. Chapter 10
  9. Chapter 11
  10. Chapter 12
  11. Chapter 13
List of Illustrations
  1. Chapter 1
  2. Chapter 3
  3. Chapter 4
  4. Chapter 5
  5. Chapter 6
  6. Chapter 7
  7. Chapter 8
  8. Chapter 9
  9. Chapter 10
  10. Chapter 11
  11. Chapter 12
  12. Chapter 13
Guide
Pages
Applied Biocatalysis
The Chemist's Enzyme Toolbox

Edited by
JOHN WHITTALL
University of Manchester Manchester
UK

PETER W. SUTTON
Glycoscience S.L. Barcelona ES

Copyright This edition first published 2021 2021 John Wiley Sons Ltd All - photo 2

Copyright

This edition first published 2021

2021 John Wiley & Sons Ltd

All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, recording or otherwise, except as permitted by law. Advice on how to obtain permission to reuse material from this title is available at http://www.wiley.com/go/permissions.

The right of John Whittall and Peter W. Sutton to be identified as the authors of the editorial material in this work has been asserted in accordance with law.

Registered Offices

John Wiley & Sons, Inc., 111 River Street, Hoboken, NJ 07030, USA

John Wiley & Sons Ltd, The Atrium, Southern Gate, Chichester, West Sussex, PO19 8SQ, UK

Editorial Office

The Atrium, Southern Gate, Chichester, West Sussex, PO19 8SQ, UK

For details of our global editorial offices, customer services, and more information about Wiley products visit us at www.wiley.com.

Wiley also publishes its books in a variety of electronic formats and by printondemand. Some content that appears in standard print versions of this book may not be available in other formats.

Limit of Liability/Disclaimer of Warranty

In view of ongoing research, equipment modifications, changes in governmental regulations, and the constant flow of information relating to the use of experimental reagents, equipment, and devices, the reader is urged to review and evaluate the information provided in the package insert or instructions for each chemical, piece of equipment, reagent, or device for, among other things, any changes in the instructions or indication of usage and for added warnings and precautions. While the publisher and authors have used their best efforts in preparing this work, they make no representations or warranties with respect to the accuracy or completeness of the contents of this work and specifically disclaim all warranties, including without limitation any implied warranties of merchantability or fitness for a particular purpose. No warranty may be created or extended by sales representatives, written sales materials or promotional statements for this work. The fact that an organisation, website, or product is referred to in this work as a citation and/or potential source of further information does not mean that the publisher and authors endorse the information or services the organisation, website, or product may provide or recommendations it may make. This work is sold with the understanding that the publisher is not engaged in rendering professional services. The advice and strategies contained herein may not be suitable for your situation. You should consult with a specialist where appropriate. Further, readers should be aware that websites listed in this work may have changed or disappeared between when this work was written and when it is read. Neither the publisher nor authors shall be liable for any loss of profit or any other commercial damages, including but not limited to special, incidental, consequential, or other damages.

Library of Congress CataloginginPublication Data

Names: Whittall, John, editor. | Sutton, Peter (Peter W.), editor.

Title: Applied biocatalysis : the chemist's enzyme toolbox / edited by Dr

John Whittall, Dr Peter W. Sutton.

Description: First edition. | Hoboken, NJ : Wiley, 2021. | Includes

bibliographical references and index.

Identifiers: LCCN 2020015374 (print) | LCCN 2020015375 (ebook) | ISBN

9781119487012 (cloth) | ISBN 9781119487029 (adobe pdf) | ISBN

9781119487036 (epub)

Subjects: LCSH: Biocatalysis.

Classification: LCC TP248.65.E59 A677 2021 (print) | LCC TP248.65.E59

(ebook) | DDC 660.6/34dc23

LC record available at https://lccn.loc.gov/2020015374

LC ebook record available at https://lccn.loc.gov/2020015375

Cover Design: Wiley

Cover Images: (Background) Angelatriks/Shutterstock,

(Inset diagram) Courtesy of GheorgheDoru Roiban


Abbreviations
a/aArea/areaAAAmino acidAADHAmino acid dehydrogenaseABTS2,2Azinobis(3ethylbenzthiazoline6sulfonic acidABSAmide bond synthetaseACNAcetonitrile (Also MECN)AcOEtEthyl acetate (Also EtOAc)ADHAlcohol dehydrogenase (Also KRED)ADPAdenosine diphosphateAEAA(S)N(2Aminoethyl)aspartic acidAHASAcetohydroxyacid synthaseAHTCAnhydrotetracyclineAMDaseAryl malonate decarboxylaseAmDHAmine dehydrogenaseAPArea percentAPIActive pharmaceutical ingredientArgKArginine kinaseArgKLPArgK from Limulus PolyphemusAroLE. coli K12 shikimate kinaseASTArylsulfotransferaseATAAmine transaminase (Also TA)ATaseAcetyl transferaseatmAtmosphere (pressure)ATPAdenosine triphosphateBCABicinchoninic acid assayBCLAgarose beads crosslinkedBABenzaldehydeBaSPSucrose phosphorylase from Bifidobacterium adolescentisBistris2[Bis(2hydroxyethyl)amino]2(hydroxymethyl)propane1,3diolBL21Competent Escherichia coli BL21BMEMercaptoethanolBMGHBuffered minimal glycerol mediumBMGYBuffered glycerolcomplex mediumBMMHBuffered minimal methanol mediumBMMYBuffered methanolcomplex mediumBnONH2OBenzylhydroxylamineBoctButoxycarbonylBSABovine serum albuminBSMBasal salt mediumBVMOBaeyerVilliger monooxygenaseBVOBaeyerVilliger oxidationcConversionCACholic acidCADCharged aerosol detectionCALBCandida antarctica lipase BCARCarboxylic acid reductaseCbzOSuN (Benzyloxycarbonyloxy)succinimideCDCAChenodeoxycholic acidCDWCell dry weightCDMOCyclododecanone monooxygenaseCFECell free extractc.f.uColonyforming unitCHMOBaeyerVilliger monooxygenase (cyclohexanone monoxygenase)CLRControlled laboratory reactorCoGCost of goodsCPCitrate phosphate (buffer)CPDCyclopentadecanoneCPMECyclopentyl methyl etherCSUCatalytic subunitCSUGSTCatalytic subunit glutathione Stransferase tagCVColumn volumeDAAODAmino acid oxidaseDAATDAmino acid transferaseDADDiodearray detectionDAPG2,4DiacetylphloroglucinolDCCDicyclohexylcarbodiimideDCMDichloromethaneddH2ODouble distilled waterDEADiethyl amineDEAEDiethylaminoethanol (group in ionexchange resin)DERA2Deoxyribose5phosphate aldolasedH2ODistilled waterDHADihydroxyacetoneDHAKDihydroxyacetone kinaseDHAPDihydroxyacetone phosphateDHIQDihydroisoquinolinedIDeoxyinosineDIDeionisedDIBALHDiisobutyl aluminium hydrideDK(R)Dynamic kinetic (resolution)DMAP4DimethylaminopyridineDMFDimethylformamideDMSODimethyl sulfoxideDNADeoxyribonucleic acidDoEDesign of experimentsDSPDownstream processingDPPA3,3Diphenylpropionic acidDTTDithiothreitolEEnantioselectivityEDDSEthylenediamineN,Ndisuccinic acidEDTAEthylenediaminetetraacetic acideeEnantiomeric excessEHEpoxide hydrolaseELSDEvaporative light scattering detectorEREnereductase (often same as ERED)EREDEnoate reductase (often same as ER)Et2ODiethyl etherEtOAcEthyl acetate (Also AcOEt)EUGOEugenol oxidaseEVExpansion vesselEWGElectronwithdrawing groupFAFormic acidFADFlavin adenine dinucleotideFADH2Flavin adenine dinucleotide reduced formFDHFormate dehydrogenaseF&FFlavour and FragranceFIDFlame ionisation detectorFMNFlavin mononucleotideFMOFlavin monooxygenase enzymeFreFlavin reductaseFSADFructose6phosphate aldolaseNext page
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